23. A nucleic acid vector for use in in vivo delivery of a nucleic acid of fascination into a targeted receiver bacterial cell, stated nucleic acid of curiosity creating a supplied impact on claimed targeted receiver bacterial mobile, whereby claimed vector comprises: claimed nucleic acid of interest, and
whereby, at the time shipped into explained specific receiver bacterial cell, said nucleic acid of interest makes reported given effect on explained targeted receiver bacterial cell when stated vector is not replicated in stated focused receiver bacterial mobile.
foundation editor molecules can also consist of two or even more of the above mentioned listed editor enzymes fused to some Cas protein (e.g. combination of an ABE and CBE). These biomolecules are named dual foundation editors and empower the modifying of two distinct bases (Grunewald et al.
The conditional origin of replication employed according to the existing creation may perhaps originate from plasmids, bacteriophages or PICIs which preferably share the next properties: they consist of in their origin of replication repeat sequences, or iterons, plus they code for at least just one protein interacting with said origin of replication (i.e. Rep, protein O, protein P, pri) which happens to be precise to them.
coli MG1655 genome following phagemid transduction in vitro employing a payload comprising a conditional origin of replication of sequence SEQ ID NO: 7, based upon a primase-helicase.
Most if possible, the genetic modification will not include both NHEJ or HR endogenous restore system on the host micro organism.
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By “host exogenous molecule expressed By natural means by other organisms” is supposed herein any molecule which isn't made by the host topic (or by a matter of exactly the same 現在贏取獎勵 species as the host species) but which is of course produced by A further organism, particularly an organism from An additional species, from One more gender, from another family members, from A further course or from another kingdom.
Plasmids carrying conditional origins of replication have a long record of use by microbiologists to be a Resource to genetically modify bacterial strains of curiosity, therefore developing secure genetically modified organisms.
in a few embodiments, focused receiver bacterial cells with the existing disclosure are anaerobic bacterial cells (e.g., cells that do not call for oxygen for progress). Anaerobic bacterial cells incorporate facultative anaerobic cells like but not limited to Escherichia coli, Shewanella oneidensis and Listeria.
notably, the quantity of vectors according to the invention, specially a vector packaged into a shipping and delivery car according to the creation, ideally a packaged plasmid or phagemid right into a bacterial virus particle based on the invention, or of the pharmaceutical or veterinary composition according to the creation, being administered must be determined by regular method well known by These of regular techniques from the art.
The current invention also issues a way for ex vivo modulating a microbiome from an setting by collecting targeted receiver bacterial cell from claimed setting and by providing a nucleic acid of interest into claimed qualified receiver bacterial cell of said microbiome, said nucleic acid of desire making a offered impact, as disclosed earlier mentioned, on explained specific receiver bacterial cell, wherein claimed approach comprises making contact with a nucleic acid vector comprising said nucleic acid of interest with reported microbiome, whereby said vector further more comprises a conditional origin of replication and that is inactive inside the focused receiver bacterial mobile but is Energetic inside a donor bacterial mobile, and explained vector is devoid of antibiotic resistance marker,
260、细菌噬菌体可选自肌尾噬菌体科(非限制性地比如以下属：cp220病毒、cp8病毒、ea214病毒、felixo1病毒、moogle病毒、susp病毒、hp1病毒、p2病毒、kay病毒、p100病毒、silvia病毒、spo1病毒、tsarbomba病毒、twort病毒、cc31病毒、jd18病毒、js98病毒、kp15病毒、moon病毒、rb49病毒、rb69病毒、s16病毒、schizot4病毒、sp18病毒、t4病毒、cr3病毒、se1病毒、v5病毒、abouo病毒、agate病毒、agrican357病毒、ap22病毒、arv1病毒、b4病毒、bastille病毒、bc431病毒、bcep78病毒、bcepmu病毒、biquarta病毒、bxz1病毒、cd119病毒、cp51病毒、cvm10病毒、eah2病毒、el病毒、hapuna病毒、jimmer病毒、kpp10病毒、m12病毒、machina病毒、martha病毒、msw3病毒、mu病毒、myohalo病毒、nit1病毒、p1病毒、pakpuna病毒、pbuna病毒、phikz病毒、rheph4病毒、rsl2病毒、rsluna病毒、secunda5病毒、sep1病毒、spn3病毒、svuna病毒、tg1病毒、vhml病毒和wph病毒)。
本发明涉及用于调节宿主微生物组的感兴趣的核酸，涉及编码所述核酸的载体以及涉及用于通过递送所述感兴趣的核酸来调节宿主微生物组的方法。